Chemical makeup of stain

* Benzene = organic compound

* Chromophore (Gk. Chroma = colour; phoros = to bear) = colour

* Auxochrome (Gk. auxein = to increase; chroma = colour) = ionization properties.

* Benzene + chromophore = chromogen

- Chromogen is a coloured compound only.

* Auxochrome with chromogen allows the dye to form salt compounds that adhere to cells.

Stain - an organic compound composed of a benzene ring, a chromophore and auxochrome group.

Benzene is a organic colourless solvent.

Chromophore is the molecule that gives colour to benzene.

(A CHROMOGEN - isn't a stain, just a colored molecule.

It is made up of the benzene and the chromophore).

Auxochrome ionizes the chromogen, gives it a charge.

This helps the chromogen form salts and bind to substances like tissues or fibers.

* Every cell as well as stain exhibit some type of charge.

* Based on charge, stain can be ACIDIC and BASIC.